Optimization of L-Citrulline Operon in Corynebacterium glutamicum for L-Citrulline Production

Reengineering of a Corynebacterium glutamicum L-arginine and L-citrulline producer.

Toward the creation of a robust and efficient producer of L-arginine and L-citrulline (arginine/citrulline), we have performed reengineering of a Corynebacterium glutamicum strain by using genetic information of three classical producers. Sequence analysis of their arg operons identified three point mutations (argR123, argG92(up), and argG45) in one producer and one point mutation (argB26 or ar...

Administration of L-arginine plus L-citrulline or L-citrulline alone successfully retarded endothelial senescence

L-citrulline and L-arginine supplementation has been shown to have several beneficial effects on the cardiovascular system. Nitric oxide (NO) protects against the progression of atherosclerosis and is synthesized by nitric oxide synthase (NOS), which converts L-arginine (L-Arg) into L-citrulline (L-Cit). Our previous study revealed that chronic administration of a combination of L-Cit and L- Ar...

Metabolic engineering of Corynebacterium glutamicum for L-serine production.

Although L-serine proceeds in just three steps from the glycolytic intermediate 3-phosphoglycerate, and as much as 8% of the carbon assimilated from glucose is directed via L-serine formation, previous attempts to obtain a strain producing L-serine from glucose have not been successful. We functionally identified the genes serC and serB from Corynebacterium glutamicum, coding for phosphoserine ...

Enhanced production of L-lysine from Corynebacterium glutamicum by mutagenesis

Optimization of Fermentation Medium for L-lysine Production by Corynebacterium Glutamicum

Corynebacterium glutamicum is an industrially important microorganism used for amino acids production. The present work describes the production of L-lysine from Corynebacterium glutamicum IIB-C9 through submerged fermentation process. Fifteen fermentation media were screened in this study for L-lysine production. Out of these fifteen media, FM:13 was found a best one for maximum production of ...